Enhancement of RNAi by a small molecule antibiotic enoxacin

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2008,（10）
• 页码范围: 1077-1079
• 页数: 3页
• 语种: 英文
• DOI: 10.1038/cr.2008.287

摘要

Dear Editor, RNAi has become a mainstream molecular tool for assessing the functions of genes in mammalian cells [1].Large-scale RNA interference-based analyses are often complicated by false positive and negative hits due to off-target effects [2] and interferon response [3],which can be attributed at least in part to the use of high concentrations of siRNA.Lowering the amounts of siRNAs and shRNAs can effectively and expediently mitigate the off-target effect and interferon response [4].