Proteomic analysis of Ions-term salinity stress-responsive proteins in Thellungiella halophila leaves

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2008,（22）
• 页码范围: 3530-3537
• 页数: 8页
• 语种: 英文
• DOI: 10.1007/s11434-008-0455-6

摘要

Salinity is one of the most severe environmental factors that may impair crop productivity. A proteomic study based on two-dimensional gel electrophoresis is performed in order to analyze the long-term salinity stress response of Thellungiella halophila, an Arabidopsis-related halophyte. Four-week-old seedlings are exposed to long-term salinity treatment. The total crude proteins are extracted from leaf blades, separated by 2-DE, stained with Coomassie Brilliant Blue, and differentially displayed spots are identified by MALDI-TOF MS or QTOF MS/MS. Among 900 protein spots reproducibly detected on each gel, 30 spots exhibit significant change and some of them are identified. The identified proteins include not only some previously characterized stress-responsive proteins such as TIR-NBS-LRR class dis-ease resistance protein,ferritin-1, and pathogenesis-related protein 5, but also some proteins related to energy pathway, metabolism, RNA processing and protein degradation, as well as proteins with un-known functions. The possible functions of these proteins in salinity tolerance of T. halophila are dis-cussed and it is suggested that the long-term salinity tolerance of T. halophila is achieved, at least partly, by enhancing defense system, adjusting energy and metabolic pathway and maintaining RNA structure.