CaSfl1 plays a dual role in transcriptional regulation in Candida albicans

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2008,（17）
• 页码范围: 2624-2631
• 页数: 8页
• 语种: 英文
• DOI: 10.1007/s11434-008-0302-9

摘要

As a newly identified transcription factor in Candida albcians, CaSfl1 has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In this study, we constructed Casfl1△/△ mutants and confirmed that deletion of this gene in-deed affected cell flocculation and filamentation. In addition, by RT-PCR we found that while Casfl1 repressed the expression of several hypha-specific genes including HWP1, ECE1, ALS1, ALS3, and FL08, it strongly activated the expression of the heat-shock protein genes HSP30 and HSP90 under certain stress conditions. Therefore, we propose that CaSfl1 can act as both positive and negative regulators, thereby playing a dual role in transcriptional controls in Candida albicans.