Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2008,（12）
• 页码范围: 1855-1860
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11434-008-0242-4

摘要

An Buthus martensii Karsch Insect Toxin (BmK IT) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1×107 PIBs/mL, the median lethal time of recombinant baculovirus (LT50=73.6 h) on third instar S. Exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50=84.8 h) during a 192 h in-fection.