Research on the effects of CD137 signaling on the function ofCD3-CD56+NK cells

• 来源期刊: 南京医科大学学报(英文版)
• 年，卷（期）: 2009,（1）
• 页码范围: 10-14
• 页数: 5页
• 语种: 英文

摘要

0bjective:To investigate the effects of CD137 signaling on the regulation of CD3-CD56+NK cells function.Methods:CD3-CD56+NK cells were treated with CD137 mAb or mouse IgG1 isotype control to study the effects of CD137 signaling on the function of CD3-CD56+NK cells.Cytotoxicity was measured by LDH activity in the supernatants of cell cultures; NKG2D and LFA-1 expression on CD3-CD56+NK cells were analyzed by flow cytometry.Results: CD137 was expressed on activated CD3-CD56+NK cells.The CD137 mAb enhanced the ability of CD3-CD56+NK cells to kill lung cancer cel]s(A549); Further studies revealed that the expression of NKG2D and LFA-1 was significantly increased in activated cells,and blockade of NKG2D and LFA-1 dramatically attenuated CD3-CD56+NK cytolysis of A549 cancer cells.Conclusion: CD137 signaling increases the ability of CD3-CD56+NK cells to kill cancer cells via up-regulating the expression of NKG2D and LFA-1.