Novel cyclen-based linear polymer as a high-affinity binding material for DNA condensation

• 来源期刊: 中国科学B辑（英文版）
• 年，卷（期）: 2009,（4）
• 页码范围: 483-488
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11426-009-0028-9

摘要

A novel cyclen-based linear polyamine (POGEC) was designed and synthesized from the reaction be-tween 1,3-propanediol diglycidyl ether and 1,7-bis(diethoxyphosphory)-1,4,7,10-tetraazacyclod- odecane.High-affinity binding between POGEC and DNA was demonstrated by agarose gel electrophoresis and scanning electron microscopy (SEM). Moreover, the formed POGEC/DNA complex (termed polyplex) could be disassociated to release the free DNA through addition of the physiological concentration of NaCl solution. Fluorescence spectrum was used to measure the high-affinity binding and DNA con-densation capability of POGEC. Circular dichroism (CD) spectrum indicates that the DNA conformation did not change after binding to POEGC.