Study of two-dimensional liquid chromatography with high temperature NPLC and room temperature RPLC

• 来源期刊: 中国高等学校学术文摘·化学
• 关键词: two-dimensional liquid chromatography HTNPLC /RPLC sample loops- valve interface traditional Chinese medicine Glycyrrhiza uralensis
• 年，卷（期）: 2009,（2）
• 页码范围: 196-201
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11458-009-0033-8

摘要

To overcome the peak band broadening and to increase the peak capacity and separation efficiency of a two-dimensional liquid chromatographic system, a high-temperature normal phase liquid chromatography (HTNPLC) was used as the first dimension (lst-D), and a RPLC was used as the second dimension (2nd-D). The sample was first separated on the lst-D CN column and the primary eluent stored in the sampling-loop system alternatively (in HTNPLC × RPLC mode) or selectively (in HTNPLC/RPLC mode) and was then transferred to 2nd-D C18 column for further separation. The resolution and separation efficiency of the systems were greatly improved. The systems were evaluated by analyzing several polycyclic aromatic hydrocarbons and Glycyrrhiza uralensis extract.