Proteome analysis of differential protein expression in infarcted rat heart after verapamil treatment

• 来源期刊: 中国高等学校学术文摘·化学
• 关键词: verapamil proteomics two-dimensional electrophoresis (2-DE) myocardial ischemia
• 年，卷（期）: 2009,（2）
• 页码范围: 202-206
• 页数: 5页
• 语种: 英文
• DOI: 10.1007/s11458-009-0020-0

摘要

To explore the protein-level mechanism of action verapamil in acute myocardial infarcted rats, the myocardial proteome was analyzed by two-dimensional electrophoresis (2-DE). Compared with the sham-operated group and the infarcted group, the result shows that 8 protein expressions in the verapamil treated group were up-regulated, and 7 protein expressions in this group were down-regulated significantly. Using MALDI-TOF-MS, 15 proteins with significant changes were identified through a database search. These proteins can be divided into 4 groups by their biological function: (1) Energy metabolism and mitochondrial function related proteins; (2) oxidative stress-induced proteins; (3) cytoskeletal Proteins; (4) other proteins. The findings show that the myocardial protective effects of verapamil in the myocardial damage process are related to the recovery of energy supply as well as anti-oxidative stress property.