Sulfonate-based fluorescent probes for imaging hydrogen peroxide in living cells

• 来源期刊: 中国科学B辑（英文版）
• 年，卷（期）: 2009,（6）
• 页码范围: 734-740
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11426-009-0109-9

摘要

Based on the mechanism of H2O2-mediated hydrolysis of sulfonates, two fluorescein disulfonates compounds (FS-1 and FS-2) were designed and synthesized as the highly selective and sensitive fluo-rescent probes for imaging H2O2 in living cells. The probes were detected with elemental analysis, IR, 1H NMR and 13C NMR. Upon reaction with H2O2, the probes exhibit strong fluorescence responses and high selectivity for H202 over other reactive oxygen species and some biological compounds. Fur-thermore, the sulfonate-based probes, as novel fluorescent reagents, are cell-permeable and can detect micromolar changes in H202 concentrations in living cells by using confocal microscopy.