Chrysanthemum indicum linné extracts attenuate the mitogenic effect of epinephrine on human HCC cells

• 来源期刊: 南京医科大学学报(英文版)
• 年，卷（期）: 2009,（4）
• 页码范围: 284-288
• 页数: 5页
• 语种: 英文

摘要

Objective: To investigate the stimulatory effect of epinephrinetEpi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells. Methods: The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with β adrenergic receptor(β 2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERKI/2 intracellular activity. Results: Epi transiently activated MAPK/ERK 1/2 in HepG2 and MHCC97H cells, resulting in a burst of growth. The effect of Epi was significantly attenuated by ICI 118551 and U0126. CILE exhibited a dose-dependent attenuation of the stimulatory effect of Epi on the growth of both cell lines and inhibited the Epi-induced activation of MAPK/ERK1/2. Conclusion: Epi, mimicking a mitogen, stimulated the growth of HepG2 and MHCC97H cells, and CILE was effective in attenuating this effect of Epi on tamor cells by inhibiting the β 2-AR-mediated activation of MAPK/ERK1/2.