Oxidative modulation of marcaine and lekoptin in H9C2 rat myoblsts

• 来源期刊: 中国药理学报(英文版)
• 关键词: heart myoblasts marcaine lekoptin oxidative stress apoptosis GST-II iNOS
• 年，卷（期）: 2009,（2）
• 页码范围: 184-192
• 页数: 9页
• 语种: 英文
• DOI: 10.1038/aps.2008.27

摘要

Aim:The cytotoxicity of marcaine was estimated in combination with a calcium channel blocker.In addition,the influence of marcaine and marcaine plus lekoptin on a model system using the H9C2 cardiac cell line was investigated.Methods:Cells were incubated for five hours with mstvsinr,lrkopytin,or with both drugs simultaneously.Apoptotic cells were detected using the TUNEL assay and the alkaline comet assay.Mitochondrial cell function after drug uptake was examined using the MTT assay.The concentration of MDA (malondialdehyde)-the final product of fatty-acid peroxi-dation,was quantified spectrophotometrically.The expression of glutathione S-transferase II(GST-II)was detected by immunofluorescence(IF)and Western blotting(WB)and inducible nitric oxide synthase(iNOS)was assessed by immuno-cytochemical staining(ABC).Results:Incubation with marcaine resulted in the marcaine resulted in the highest number of apoptotic cells.After incubation with both marcaine and lekoptin,moderate damage to cells(54.2%±1/775% of DNA destruction)was observed.The characteristic nuclear GST-II expression were observed in cells treated with both drugs.Conclusion:Lekoptin stimulated cells to proliferate.Marcaine caused membrane damage and ultimately cell death.