A facultative thermophilic alkaline desulphuricant strain named GDJ-3 was isolated from neutral soils, enriched on sulfur synthetic medium, and detected in previous work. Conventional and chemotax-onomic analyses and 16s RNA gone sequencing showed that the strain was in good agreement with Alpha proteobacterium sp. (97%) and ochrobactrum sp. (98%). The regenerative processes of the solu-tion containing sulfur compounds (SCS) from the strain through an orthogonal test were investigated to get the optimum regenerative condition. The results showed that regenerative temperature, air flow, and stirring speed of the agitator were the main three variables influencing the regenerative processes of the SCS. The optimum regenerative efficiency of the SCS from the strain was obtained when tem-perature, air flow, and stirring speed of the agitator were 318.2 K, 3.0 L/min, and zero r/min, respectively. Under this condition, when the cell concentration of the strain was adjusted to 107/mL, the concentra-tions of Na2S2O3 and Na2S in the SCS decreased from 112.68 g/L to 96.88 g/L and from 0. 87 g/L to 0.11 g/L in 9.5 h. Meanwhile, XRD spectrum shows that sulfur was formed in the regeneration process. These results suggest that the strain has potential application to the regeneration of the industrial so-lution containing sulfur compounds.