Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase

• 来源期刊: 中国科学B辑（英文版）
• 年，卷（期）: 2009,（6）
• 页码范围: 827-832
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11426-009-0073-4

摘要

The cationic charged water-soluble polyfluorenee containing 2,1,3-benzothiadiazole (BT) units (P1--3) have been synthesized and characterized. These polymers demonstrate intramolecular energy transfer from the fluorene units to the BT sites when oppositely charged hyaluronan is added due to the forma-tion of electrostatic complexes, followed by a shift in emission color from blue to green or brown. Upon adding hyaluronidaee, the hyaluronan is cleaved into fragments. The relatively weak electrostatic in-teractions of hyaluronan fragments with polyfluorenes keep their main chains separated and energy transfer from the fluorene units to the BT sites is inefficient, and the polyfluorenes recover their blue emissions. The complexes of conjugated polymers/hyaluronan can be utilized as probes for sensitive and facile fluorescence assays for hyaluronidase. The new assay method interfaces with the aggrega-tion and light harvesting properties of conjugated polymers.