Characterization of catechol 1,2-dioxygenase from cell extracts of Sphingomonas xenophaga QYY

• 来源期刊: 中国科学B辑（英文版）
• 年，卷（期）: 2009,（5）
• 页码范围: 615-620
• 页数: 6页
• 语种: 英文
• DOI: 10.1007/s11426-008-0149-6

摘要

Sphingomonas xenophaga QYY, capable of growing significantly on more than ten kinds of aromatic compounds as sole carbon source, was used to study characterization of catechol 1,2-dioxygenase (C120) in cell extracts. Characterization of the crude C120 showed that the maximum activity was obtained at 40-70℃ and pH 7.8-8.8. Metal ions had different influences on the activity of crude C120. It was suggested that strain QYY possessed an inducible and ferric-dependent C120. Kinetic studies showed that the value of Vmax and Km was 0.25 μmol catechol/L/mg protein/min and 52.85 μmol/L, respectively. In addition, the partial purification of C120 was achieved by a HiTrap Q Sepharose column chromatography.