Double knockouts in human embryonic stem cells

• 来源期刊: 细胞研究(英文版)
• 年，卷（期）: 2010,（3）
• 页码范围: 250-252
• 页数: 3页
• 语种: 英文
• DOI: 10.1038/cr.2010.29

摘要

Gene targeting is the method of pre-cise genome modification that utilizes the innate DNA repair mechanism in cells, known as homologous recom-bination. At its genesis, gene targeting in mouse embryonic stem (ES) cells was a breakthrough for genetics and developmental biology. Its application has enhanced researchers' ability to study gene function and model human disease, which was recognized by the Nobel Prize in Physiology or Medicine 2007.