An imprinted signature helps isolate ESC-equivalent iPSCs

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2010,（9）
• 页码范围: 974-976
• 页数: 3页
• 语种: 英文
• DOI: 10.1038/cr.2010.117

摘要

Since the demonstration of direct reprogramming of differentiated cells such as fibroblasts, to a malleable, pluri-potent state by defined transcription fac-tors great effort has been given to isolate induced pluripotent stem cells (iPSCs) with the same developmental potential as embryonic stem cells (ESCs) derived from blastocysts. Various selection and morphological criteria have led to the isolation of iPSCs with differential pluripotent capacity, but without the addition of small molecules very few lines have been able to undergo the most stringent pluripotent test - generate vi-able "all iPS cell mice" by tetraploid complementation. In a recent elegant study, Stadtfeld and colleagues have proposed that silencing of the imprinted Dlkl-Dio3 gene cluster is responsible for this variability of pluripotency po-tential in iPSCs, and the key to isolating ESC equivalent iPSCs [1].