Preparation and evaluation of an enzymatic microreactor based on HILIC matrix for digestion and identification of proteins

• 来源期刊: 科学通报(英文版)
• 学科: 生物学
• 关键词: 酶催化反应 动态矩阵 蛋白质 评价鉴定 胰蛋白酶活性 酶固定化 消化 制备
• 年，卷（期）: 2010,（10）
• 所属期刊栏目: ARTICLES
• 页码范围: 910-914
• 页数: 5页
• 分类号: Q55 TD921.5
• 语种: 英文

摘要

In the present study,a new enzymatic microreactor is developed by immobilizing trypsin on the matrix-HILIC (hydrophilic interaction chromatography).The influences of different types of buffer solutions (borate,Tris?HCl,and phosphate),their concentrations and pH values on the immobilizing rate and the activity of immobilized trypsin were investigated.The optimal condition for off-line immobilization of trypsin on matix-HILIC was Tris?HCl buffer (20 mmol/L,pH 8.0),and then,under the optimal condition,an enzym...