Stable expression of Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1, and salt tolerance in transgenic soybean for over six generations

• 来源期刊: 科学通报(英文版)
• 学科: 生物学
• 关键词: 转基因大豆 稳定表达 蛋白基因 逆向转运 耐盐性 液泡膜 拟南芥 Northern杂交
• 年，卷（期）: 2010,（12）
• 所属期刊栏目: ARTICLES
• 页码范围: 1127-1134
• 页数: 8页
• 分类号: Q785 S565.1
• 语种: 英文

摘要

The Arabidopsis vacuolar Na+/H+ antiporter gene,AtNHX1,was introduced into soybean by Agrobacterium-mediated transformation.Four independent kanamycin resistant lines were obtained.The result of PCR,Southern blotting and Northern blotting analyses demonstrated that the AtNHX1 gene was successfully inserted into the soybean genome and stably expressed in these kanamycin resistant lines.The stability of AtNHX1 expression and salt resistance were evaluated in the soybean transformants for over 6 generations.Tw...