Targeted conditional gene knockout in human embryonic stem cells

• 来源期刊: 细胞研究(英文版)
• 年，卷（期）: 2010,（3）
• 页码范围: 379-382
• 页数: 4页
• 语种: 英文
• DOI: 10.1038/cr.2010.23

摘要

Dear Editor, We report our application of a promoterless knockout(KO) strategy combined with Flpase- and Cre-mediated recombination to efficiently ablate gene function in hu-man embryonic stem (hES) cells. Using this strategy, we modified and deleted both alleles of BAF250a (ARID1A), a signature component of the ATP-dependent SWI/ SNF chromatin remodeling complex BAF. This strategy should facilitate the functional studies of genes essential for hES cell self-renewal and differentiation, and be in-strumental for human therapeutic applications.