The permeability effect of microcystin-RR on Escherichia coli and Bacillus subtilis

• 来源期刊: 科学通报(英文版)
• 学科: 生物学
• 关键词: 埃希氏大肠杆菌 枯草芽孢杆菌 微囊藻毒素 膜通透性 居民 微生物细胞 浓度增加 细胞通透性
• 年，卷（期）: 2010,（18）
• 页码范围: 1894-1898
• 页数: 5页
• 分类号: Q936 S858.335.1
• 语种: 英文

摘要

Microcystins are a kind of cyclic hepatoxins produced by many species of cyanobacteria.Most previous work have been done on the toxic effects of microcystins on animals and plants.However,the reports about the effect of microcystins on microbial cells are very limited.In this work,the permeability of MC-RR on the cell outer membrane of Escherichia coli(E.coli) and Bacillus subtilis(B.subtilis) was discussed.The permeability effect of MC-RR on the cell outer membrane of E.coli and B.subtilis under different concentrations was demonstrated by a rapid and sustained reduction in the A675 values of lysozyme-treated cells.The decrease of the absorbance values showed a time-and dose-effect.The extravasations of protein and carbonhydrate increased with the increment of the treated-concentration of MC-RR.The results showed that MC-RR could increase the permeability of cell outer membranes of E.coli and B.subtilis.The synergistic effects of MC-RR and lysozyme on bacteria indicated that MC-RR might play an ecological role in bacteria in combination with other substances in some aquatic environments.