Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing

• 来源期刊: 科学通报(英文版)
• 学科: 农学
• 关键词: 细菌人工染色体 基因组测序 普通小麦 应用 排序 BAC文库 物理图谱 基因组大小
• 年，卷（期）: 2010,（15）
• 所属期刊栏目: REVIEW
• 页码范围: 1463-1468
• 页数: 6页
• 分类号: S512.1 Q78
• 语种: 英文

摘要

The large genome size (～17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing.By means of flow cytometry,systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s.Several wheat chromosome-specific BAC libraries,such as chromosome 3B,three D genome chromosomes (1D,4D and 6D),and the short arm of chromosome 1B,have been developed,and the ph...