透镜上的 FGFR1 表示的比较在成年人和胎儿之间的上皮的房间

• 作者: Shu-Ling Peng Yu-Fu Liu,
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: human LENS EPITHELIAL cells FIBROBLAST growth factor receptor 1 indirect in SITU RT-PCR after-cataract
• 年，卷（期）: 2011,（1）
• 页码范围: 37-39
• 页数: 3页
• 分类号: R77

摘要

AIM:To study the differences of fibroblast growth factor receptor 1(FGFR1) gene on human lens epithelial cells(HLECs) of adults and fetuses.METHODS:Indirect in situ RT-PCR was adopted for detection of FGFR1 gene.The cDNA of the mRNA in the paraffin sections of fetus and adult HLEC was synthesized by reverse transcription reaction.After PCR amplification,in situ hybridization test was performed with synthesized oligonucleotide probe and relative quantification was carried out using image analysis.RESULTS:HLECs of adults and fetuses expressed FGFR1 gene,the expression level was higher in fetuses than in adults.The difference between them had significance(P 【0.05).CONCLUSION:FGFR1 exist in HLEC and the expression is age-related,which could be one of causes of the high occurrence of post operational after-cataract in children.