Bone marrow increases human islets insulin positive cells in co-culture: quantification with flow cytometry

• 作者: Fang Xiong John Z. Q. Luo LuGuang Luo Mary Hassani Zhengke Wang
• 来源期刊: 糖尿病(英文)
• 学科: 医学
• 关键词: Bone MARROW Human ISLETS Flow CYTOMETRY
• 年，卷（期）: 2011,（4）
• 页码范围: 109-117
• 页数: 9页
• 分类号: R73

摘要

We have previously demonstrated that allogeneic human bone marrow (BM) supports human islet function and longevity in vitro. We hypothesize that BM supporting human islets may include to increase β-cell in cultured islets. In this study, we developed a method to quantify insulin-producing β cells from cultured islets by using immunofluorescent staining and flow cytometry analysis to explore this possibility. The results show that human islets cocultured with BM for 39 days contained a significantly higher number of insulin-positive β cells (42.3% ± 4.5%) compared to the islet-only cultures (1.15% ± 0.78%), and increased insulin release levels evaluated by ELISA is consistent with increased β cells in same culture condition. Human islet culture with BM significantly increase β-cells while islet only culture lost β-cells in same culture period supports the possibility of BM increasing β-cells in cultured islets.