Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2011,（11）
• 页码范围: 1638-1640
• 页数: 3页
• 语种: 英文
• DOI: 10.1038/cr.2011.153

摘要

Dear Editor,Gene targeting is in widespread use as a gold standard for determining the function of genes in mice and human embryonic stem cells [1].However,the poor efficiency of this technology has hindered its application to domestic animals,for which embryonic stem cells are not available.Although gene-targeted large domestic animals have been produced successfully by combination of homologous recombination-based targeting strategy and cloning [2-4],the efficiency is very low and,more importantly,the disruption of the targeted gene is usually mono-allelic.It thus takes a long time to obtain a null mutant.