Construction of recombinant adenovirus vector with connexin 43 gene

• 来源期刊: 生物化学与生物物理学报（英文版）
• 年，卷（期）: 2012,（10）
• 页码范围: 894-895
• 页数: 2页
• 语种: 英文
• DOI: 10.1093/abbs/gms069

摘要

Biological therapy is one of the essential cancer treatments [1].The key issues of gene therapy are:whether the right carrier could be chosen,determining if the foreign genes were transfected into target cells stably and expressed efficiently.Adenovirus is an ideal carrier [2].This recombinant replication-defective adenovirus vector (pAd/CMV/V5-DEST) could be used for gene therapy in tumors and has vast improvements compared with the previous generation of adenovirus vector [3].There are lots of advantages to transfect with this vector both in vitro and in vivo,such as high transfection efficiency,low toxicity,weak immunogenicity,and long periods of gene product expression.This vector could ensure the safety of the transfected cells [4].