Structural and functional topography of the human ribosome

• 来源期刊: 生物化学与生物物理学报（英文版）
• 关键词: human ribosome photoaffinity crosslinking mRNA and tRNA binding site hepatitis C IRES binding site ribosomal component
• 年，卷（期）: 2012,（4）
• 页码范围: 281-299
• 页数: 19页
• 语种: 英文
• DOI: 10.1093/abbs/gmr118

摘要

This review covers data on the structural organization of functional sites in the human ribosome,namely,the messenger RNA binding center,the binding site of the hepatitis C virus RNA internal ribosome entry site,and the peptidyl transferase center.The data summarized here have been obtained primarily by means of a site-directed crosslinking approach with application of the analogs of the respective ribosomal ligands bearing cross-linkers at the designed positions.These data are discussed taking into consideration available structural data on ribosomes from various kingdoms obtained with the use of cryo-electron microscopy,X-ray crystallography,and other approaches.