Isolation Technology of Single Spore and Optimization of Conidia Culture Condition for Ustilaginoidea virens

• 作者: Haiyong HE Jie YUAN Lishuang WANG Qingqun TAN Shiping WU WongkaewSopone Xiaojun CHEN Xuehui YANG
• 来源期刊: Plant Diseases and Pests(植物病虫害研究：英文版)
• 学科: 农学
• 关键词: 最佳培养条件 分生孢子 稻曲病菌 隔离技术 单孢子 优化 孢子培养 自然光
• 年，卷（期）: 2012,（5）
• 页码范围: 21-23
• 页数: 3页
• 分类号: S476.12

摘要

The single spores were isolated from chlamydospores of Ustilaginoidea virens with three different maturities by PSA. The isolated single spores were cultured on different media at different temperatures under natural light for inducing conidia to explore the optimum isolation technique of single spore and optimum culture condition of conidia. The results showed that the successful isolating rate of single spore from yellow rice false smut balls reached 90. 00% . The sporulation quantities of isolated single spores cultured on PSD and PDB media at 22 - 29 ℃ ( variable temperature under natural light) or 28 ℃ ( constant temperature under dark condition) for 12 d were up to 6. 3 × 107 and 1. 1 × 106 spore / mL,respectively. PSA was the most effective method to isolate single spores from yellow rice false smut balls of U. virens. The optimum conidia culture condition included PSD or PDB medium,22 - 29 ℃ or 28 ℃ ,natural light and vibration culture.