Lagging chromosomes entrapped in micronuclei are not ‘lost' by cells

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2012,（5）
• 页码范围: 932-935
• 页数: 4页
• 语种: 英文
• DOI: 10.1038/cr.2012.26

摘要

Dear Editor, In the literature,chromosomes lagging during anaphase and entrapped in micronuclei are considered to be a sign of genetic material loss and closely associated with aneuploidy formation [1-4].However,some of these assumptions were based on analysis of fixed cells,and the actual behaviors of lagging chromosomes and micronuclei in live cells are poorly characterized [5].To avoid the shortcomings associated with fixed samples,we previously labeled all the chromosomes with fluorescent protein for live cell imaging and have provided preliminary data on the formations of lagging chromosomes,micronuclei and their effect on the fate of cells [6,7].Unfortunately,labeling of all chromosomes prevents us from precisely tracking the fates of lagging chromosomes and micronuclei [7].To address these issues,new experimental strategies are required.