Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS

• 作者: Jong-Shik Kim
• 来源期刊: 农业化学和环境(英文)
• 学科: 医学
• 关键词: POLYCHLORINATED BIPHENYL (PCB) CARVONE RHODOCOCCUS Protein Expression
• 年，卷（期）: 2013,（3）
• 页码范围: 65-73
• 页数: 9页
• 分类号: R73

摘要

Protein expression patterns in the polychlorinated biphenyl (PCB)-degrading Rhodococcussp. strain ACS were examined following growth on two substrates capable of inducing the enantioselective biotransformation of PCBs via different degradation pathways. Eleven inducible proteins were identified by SDS-PAGE and characterized by LC-MS/MS. Four of the peptides, a spore coat protein, an extracellular serine protease, a spoVP, and a molecular chaperonin from Bacillus subtilis, were identified as being unique to biphenyl-induced cells, whereas anextracellular serine protease from B. subtilis was identified as being unique to carvone-induced cells. None of the peptides identified had sequences that corresponded to known dioxygenases or other PCB-degrading enzymes of this Gram- positive bacterium, suggesting that the identified induced proteins may be involved in either PCB degradation or adaptive responses that protect cells from toxicity.