Detecting Concentration of Analytes with DETECHIP: A Molecular Sensing Array

• 作者: Andrea E. Holmes Gary Batres Hannah Johnke Mark Wilson Sharmin Sikich
• 来源期刊: 传感技术(英文)
• 学科: 医学
• 关键词: COLORIMETRIC ARRAYS SENSORS ANALYTE CONCENTRATION RGB Analysis Drug Detection
• 年，卷（期）: 2013,（3）
• 页码范围: 94-99
• 页数: 6页
• 分类号: R73

摘要

DETECHIP is a detection system made of various sensors that has been shown to detect and discriminate between small molecules of interest, including various illicit and over-the-counter drugs. Previously, detection was normalized to a single concentration of analyte. Now this detection assay can detect concentration differences in analytes via red, green, and blue color value changes and shifts in the UV-Vis spectra of the assay. To determine the concentrations differences, the exposed assays were scanned on a flatbed scanner and the images were analyzed for individual RGB values with a custom macro in ImageJ, an image analysis program. Increasing concentrations of the analyte resulted in greater differences in color values between control and analyte wells. These differences showed a linear relationship to concentration change, some with correlation coefficients greater than 98%. This work expands the capability of DETECHIP to give information about the concentration of analyte when the analyte identity is known.