Purification, Crystallization and Preliminary X-Ray Diffraction Analysis of Exodeoxyribonuclease III from Crenarchaeon <i>Sulfolobus tokodaii</i>Strain 7

• 作者: Chieko Naoe Kazuo T. Nakamura Masaru Tsunoda Shuichi Miyamoto
• 来源期刊: 晶体结构理论与应用(英文)
• 学科: 化学
• 关键词: Crenarchaeon CRYSTALLIZATION Exodeoxyribonuclease SULFOLOBUS TOKODAII X-Ray DIFFRACTION
• 年，卷（期）: 2013,（4）
• 页码范围: 155-158
• 页数: 4页
• 分类号: O6

摘要

Exodeoxyribonuclease III (EXOIII) acts as a 3’→5’ exonuclease and is homologous to purinic/apyrimidinic (AP) endonuclease (APE), which plays an important role in the base excision repair pathway. To structurally investigate the reaction and substrate recognition mechanisms of EXOIII, a crystallographic study of EXOIII from Sulfolobus tokodaii strain 7 was carried out. The purified enzyme was crystallized by using the hanging-drop vapor-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 154.2, b = 47.7, c = 92.4 ?, β = 125.8° and diffracted to 1.5 ? resolution.