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摘要:
Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of a reporter gene (d2EGFP) has been used to analyse the role of certain defined putative elements and a downstream enhancer element in gene expression. These experiments were carried out in vitro using a line of pre-pubertal mouse Sertoli cells, transienly transfected with circular DNA constructs with variously mutated promoter elements. A downstream enhancer element, situated immediately 3’ of the polyadenylation (PA) signal for Amh, has been inserted in an equivalent position in the d2EGFP construct. When the Amh promoter is unmodified, the downstream enhancer (DE) is positively associated with a large increase in EGFP expression. This is at least partly the consequence of an increased rate of expression by individual cells. Experiments using variously truncated Amh promoters indicate that an upstream region (-214 to -336) may play a minor role in facilitating enhancement. However mutation of the Wilms tumour factor-1 element, situated between the tata box and the start of translation, results in an almost complete suppression of enhancement.
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篇名 Interaction between the Wilms tumour factor-1 element in the promoter of Amh and a downstream enhancer is required for a strong expression of the gene in pre-pubertal sertoli cells
来源期刊 美国分子生物学期刊(英文) 学科 医学
关键词 Mouse Cell Lines In Vitro AMH PROMOTER SMAT Pre-Pubertal Sertoli DOWNSTREAM ENHANCER Wilms Tumour Factor ELEMENT
年,卷(期) 2013,(3) 所属期刊栏目
研究方向 页码范围 165-172
页数 8页 分类号 R73
字数 语种
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Mouse
Cell
Lines
In
Vitro
AMH
PROMOTER
SMAT
Pre-Pubertal
Sertoli
DOWNSTREAM
ENHANCER
Wilms
Tumour
Factor
ELEMENT
研究起点
研究来源
研究分支
研究去脉
引文网络交叉学科
相关学者/机构
期刊影响力
美国分子生物学期刊(英文)
季刊
2161-6620
武汉市江夏区汤逊湖北路38号光谷总部空间
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191
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