A Fluorescence Ratiometric Probe for Cysteine/Homocysteine and Its Application for Living Cell Imaging

• 作者: Lin Wang Lingliang Long Yanjun Wu
• 来源期刊: 有机化学国际期刊(英文)
• 学科: 医学
• 关键词: FLUORESCENT Probes FLUORESCENCE CYSTEINE and HOMOCYSTEINE FLUORESCENCE IMAGING
• 年，卷（期）: 2013,（4）
• 页码范围: 235-239
• 页数: 5页
• 分类号: R73

摘要

A fluorescence ratiometric probe 1 for cysteine (Cys) and homocysteine (Hcy) has been rationally constructed based on intramolecular charge transfer (ICT) mechanism. Upon treatment with Cys/Hcy, probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength displaying a large shift (from 526 nm to 446 nm). When 90 μM Cys were added, the emission ratios (I446/I526) of the probe changed dramatically from 0.01797 to 4.65472. The detection limit was also measured to be 0.18 μM (S/N = 3). The theoretical calculations have confirmed that the ratiometric response of probe 1 to Cys/Hcy is due to the inhibition of ICT process upon the reaction of probe 1 with Cys/Hcy. Furthermore, the fluorescence imaging experiments in living cell demonstrated that probe 1 was favourable for intracellular Cys/Hcy imaging.