Comparison of TGF-β₁ in tears and corneal haze following Epi-LASIK with and without mitomycin C

• 作者: Jing Chen Su-Ning Han Yi Chen
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: EPI-LASIK MMC TGF-β1 HAZE
• 年，卷（期）: 2013,（3）
• 页码范围: 312-315
• 页数: 4页
• 分类号: R779.63

摘要

AIM: To compare transforming growth factor-β1 (TGF- β1 ) levels in tears and the degree of corneal haze formation following epithelial laser in situ keratomileusis (Epi -LASIK) with and without the use of mitomycin C (MMC) and to investigate the effect of MMC on corneal wound healing. ·METHODS: Thirty-two patients (64 eyes) with high myopia underwent Epi -LASIK surgery, and MMC was randomly used in one eye in each patient. The epithelialization process was observed, and the TGF -β1 level in tears was measured at 1 day, 3, and 7 days postoperatively for comparison with baseline. Corneal haze was graded at 1 month, 3, and 6 months after surgery. · RESULTS: Mean preoperative spherical equivalent refraction was -8.24 ±2.18D (range-6.00 to -10.50D) in the MMC group and -7.82±1.55D (range -6.00 to -9.75D) in the non -MMC group. There was no significant difference between the two groups (P =0.38). Mean epithelialization time was (5.02 ±0.68) days in the MMC group and (4.86±0.57) days in the non-MMC group (P = 0.31). Tear fluid TGF-β1 levels were similar before surgery (P =0.34), but were significantly higher in the non-MMC group at 1 day, 3, and 7 days postoperatively (P =0.004, 0.008, and 0.012, respectively). Corneal haze scores 1 month after surgery were significantly higher in the non -MMC group (P =0.03), and similar at 3 and 6 months after surgery (P =0.28 and 0.62, respectively). ·CONCLUSION: MMC did not delay epithelialization. Inearly postoperative period, lower TGF -β1 levels in tears and a lower grade of corneal haze were observed in the MMC group. Our findings suggest that the ability of MMC to inhibit Epi -LASIK -induced haze might be mediated through TGF-β1 suppression.