Selection of reference genes for quantitative gene expression studies in the house fly (Musca domestica L.) using reverse transcription quantitative real-time PCR

• 来源期刊: 生物化学与生物物理学报（英文版）
• 年，卷（期）: 2013,（12）
• 页码范围: 1069-1073
• 页数: 5页
• 语种: 英文
• DOI: 10.1093/abbs/gmt111

摘要

Relative gene expression analysis is highly important in many fields of biological research.Understanding the expression pattem of genes provides the highest probability of identifying genes relevant to new biological processes,and gene expression pattems provide a useful way to study complex regulatory networks.The relative quantification of mRNA levels by reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a technique that is currently used extensively.Reliable quantification depends on the selection of one or more stably expressed reference gene(s) (RGs),which are usually housekeeping genes,as intemal controis.They include genes encoding products with functions that maintain the cell-wall structure and the primary metabolism,such as 18S ribosomal RNA (18S),actin (ACT),tubulin (TUB),glyceraldehyde-3-phosphate dehydrogenase (GAPDH),polyubiquitin (UBQ),and elongation factor-1 (EF-1) [1-3].