词法并且在 vitro 的人的网膜的神经胶质子类型的 immunocytochemical 分析

• 作者: Bin Li Shao-Fen Lin Shi-Bo Tang Wei Sun Yu-Xiang Mao
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: RETINA GLIA morphology
• 年，卷（期）: 2013,（5）
• 页码范围: 559-563
• 页数: 5页
• 分类号: R774.1

摘要

AIM:To examine the morphological characteristics and antigen expression patterns of cultured human retinal glia to define novel subtypes.METHODS:Morphologic characteristics and marker expression were examined during cultivation using hematoxylin and eosin(HE) and immunostaining for glial fibrillary acidic protein(GFAP) and vimentin.RESULTS:A subtype of human retinal glia distinct from radial glia(Müller cells) was successfully isolated by digesting the retina first in diastase vera(pancreatin) and then in clostridiopeptidase,followed by culture on fibronectin substrate in human endothelial cell medium(supplemented with 10% fetal bovine serum,growth factors,and heparin sodium).Adherence was detected at 72h and cell-cell coupling at 9d-10d after seeding.These cells were extensively and strongly immunopositive for GFAP and vimentin,consistent with glial expression patterns in the human retina,but were morphologically and immunohistochemically distinct from previously reported cultured retinal glia,including GFAP-positive and glutamine synthetase(GS)-positive Müller cells.CONCLUSION:A unique human retinal glial cell type can be isolated using diastase vera and clostridiopeptidase and then maintained in vitro.Further studies are required to characterize the physiological and pathological functions of these cells.