Measurement of the binding force between RAS protein and a pathologic BRAF mutant using optical tweezers

• 来源期刊: 中国光学快报（英文版）
• 年，卷（期）: 2013,（9）
• 页码范围: 80-83
• 页数: 4页
• 语种: 英文
• DOI: 10.3788/COL201311.091702

摘要

Activating mutants in rat sarcoma (RAS) and B-rapid accelerated fibrosarcoma (BRAF) are found in at least a third of cases of human tumors and melanoma;hence,numerous therapeutic treatments target this pathway.In this letter,we study the adhesion force of RAS-coated beads with BRAF-coated beads,BRAF (A246P) mutant-oated beads,and GST-coated beads using optical tweezers.One full and two fractional RAS-BRAF specific binding modes are identified using the rupture force distribution.The koff(0) of the full binding mode in RAS BRAF is 3.71×10-4/s and 1.16× 10-4s-1 in RAS-BRAF (A246P),whereas the xb is around 3×10-10 m in both groups.