ACE-I inhibitory peptide fractions from enzymatic hydrolysates of velvet bean (<i>Mucuna pruriens</i>)

• 作者: Carlos Paul Espadas-Alcocer David Betancur-Ancona Luis Chel-Guerrero Maira Rubi Segura-Campos
• 来源期刊: 农业科学(英文)
• 学科: 医学
• 关键词: VELVET BEAN MUCUNA pruriens ACE-I Inhibition Peptide FRACTIONS Enzymatic Hydrolysis
• 年，卷（期）: 2013,（12）
• 页码范围: 767-773
• 页数: 7页
• 分类号: R73

摘要

The hydrolysis of velvet bean (Mucuna pruriens) protein in the presence of Alcalase?-Flavourzyme? and Pepsin-Pancreatin was investigated. The results showed that Alcalase?-Flavourzyme? (29.08%) sequential system catalyzed the hydrolysis most efficiently that Pepsin-Pancreatin (24.78%). In addition, the higher ACE-I inhibitory activity was achieved with the sequential system Alcalase?-Flavourzyme? (33.13%). Furthermore, the concentration of peptides employing an ultrafiltration (UF) system or their purification by gel filtration chromatography showed that the oligomeric peptides with lower molecular weight registered the highest ACE-I inhibitory activity. It has been demonstrated that Mucuna pruriens protein hydrolysates could serve as a source of peptides with ACE inhibitory activity and this activity can be attributed mainly to the mixture of short peptides in the hydrolysate.