The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2013,（12）
• 页码范围: 1414-1416
• 页数: 3页
• 语种: 英文
• DOI: 10.1038/cr.2013.146

摘要

Dear Editor, Insect genome engineering is crucial for gene functional analysis and the long-term development of novel pest control technologies.The recently developed engineered nucleases,zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs),are capable of facilitating precise genome modifications [1].However,constructing separate nucleases for each targeting site is time-and labor-consuming,especially for ZFNs.The recently identified CRISPR/Cas system,which is involved in an adaptive immune mechanism found in many bacterial and archaeal species,is a facile and efficient alternative to ZFNs and TALENs.Of the 4 known CRISPR/Cas systems,the type Ⅱ bacterial CRIS-PR/Cas system is most suitable for genome engineering [2].The Cas9 endonuclease from the Streptococcus pyogenes type Ⅱ system cleaves DNA under the guidance of a synthetic single guide RNA (sgRNA) [3].Indeed,the Cas9-sgRNA complex has been used in several organisms to introduce mutations at single or multiple sites [4-6].