Piglets cloned from induced pluripotent stem cells

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2013,（1）
• 页码范围: 162-166
• 页数: 5页
• 语种: 英文
• DOI: 10.1038/cr.2012.176

摘要

Dear Editor, Embryonic stem (ES) cells are powerful tools for generating genetically modified animals that can assist in advancing our knowledge of mammalian physiology and disease.Pigs provide outstanding models of human genetic diseases due to the striking similarities to human anatomy,physiology and genetics,but progress with porcine genetic engineering has been hampered by the lack of germline-competent pig ES cells.To overcome this limitation,genetically modified pigs have been produced using genetically modified somatic cells and nuclear transfer (NT).Yet,somatic cells exhibit limited proliferative capacity and have an extremely low frequency of homologous recombination compared to ES cells.Hence,only a few knockout pig models have been reported thus far using standard gene-targeting approaches.