ARB affects nicotine-induced gene expression profile in human coronary artery endothelial cells

• 作者: Akiko Saito-Hakoda Akira Sugawara Akira Uruno Atsushi Yokoyama Ikuko Sato Kaori Sugawara Ken Matsuda Kyoko Shimizu Masataka Kudo Naotaka Kogure Ryo Ito Sadayoshi Ito Takako Saito-Ito Takeo Yoshikawa Yuko Iki
• 来源期刊: 世界高血压杂志
• 学科: 医学
• 关键词: AngiotensinⅡ Atherosclerosis MICROARRAY OLMESARTAN SMOKING
• 年，卷（期）: sjgxyzz_2014,（1）
• 页码范围: 7-14
• 页数: 8页
• 分类号: R

摘要

AIM:To investigate the effects of nicotine and nicotine plus angiotensinⅡreceptor blocker(ARB)on the gene expression profile of human coronary artery endothelial cells(HCAECs).METHODS:The changes in gene expression profiles in HCAECs treated with nicotine and nicotine plus ARB olmesartan were analyzed by DNA microarray.In nicotine-treated HCAECs,432 genes selected by P<0.01 were greater than 1.5-fold compared with the untreated cells.Data were analyzed using IPA(Ingenuity?Systems,www.ingenuity.com).RESULTS:The gene expression levels of tumor necrosis factor-?collagen type 1,matrix metalloproteinase-10,and disintegrin and metalloprotease domain 8,which are related to揷ardiovascular function and disease?were significantly increased.In canonical pathway analyses using IPA,揳therosclerosis signaling?was strongly affected by nicotine treatment and this effect was reduced by co-incubation with ARB olmesartan.These data indicate that the deleterious cardiovascular consequences of cigarette smoking may,at least in part,be due to the nicotine-induced gene expression profile related to揳therosclerosis signaling?CONCLUSION:The inhibitory effect of ARB against the nicotine-induced gene expression profile may possibly induce anti-atherosclerotic effects that are independent of those from lowering the blood pressure.