Review of application of mass spectrometry for analyses of anterior eye proteome

• 作者: Ashley M Crane Michael Margolis Sanjoy K Bhattacharya Sherif Elsobky Teresia A Carreon
• 来源期刊: 世界生物化学杂志：英文版（电子版）
• 学科: 化学
• 关键词: Mass spectrometry PROTEOMICS OCULAR GLAUCOMA
• 年，卷（期）: 2014,（2）
• 页码范围: 106-114
• 页数: 9页
• 分类号: O657.63

摘要

Proteins have important functional roles in the body, which can be altered in disease states. The eye is a complex organ rich in proteins; in particular, the anterior eye is very sophisticated in function and is most commonly involved in ophthalmic diseases. Proteomics, the large scale study of proteins, has greatly impacted our knowledge and understanding of gene function in the post-genomic period. The most significant breakthrough in proteomics has been mass spectrometric identification of proteins, which extends analysis far beyond the mere display of proteins that classical techniques provide. Mass spectrometry functions as a 'mass analyzer' which simplifies the identification and quantification of proteins extracted from biological tissue. Mass spectrometric analysis of the anterior eye proteome provides a differential display for protein comparison of normal and diseased tissue. In this article wepresent the key proteomic findings in the recent literature related to the cornea, aqueous humor, trabecular meshwork, iris, ciliary body and lens. Through this we identified unique proteins specific to diseases related to the anterior eye.