Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (<i>WT</i>1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry

• 作者: Marie Olszewski Morris Kletzel Sana Khan Wei Huang
• 来源期刊: 生命科学与技术进展(英文)
• 学科: 医学
• 关键词: JUVENILE Myelo-Monocytic Leukemia WILMS Tumor Nested PCR JMML WT1 GM-CSF nPCR
• 年，卷（期）: 2014,（2）
• 页码范围: 155-159
• 页数: 5页
• 分类号: R73

摘要

This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71+CD34+ in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene expression and cellular proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.