Clinical Value of Molecular Biological Methods in Respiratory Tuberculosis in Children

• 作者: Ulia Ovchinnikova Anna Starshinova Irina Dovgalyuk Natalia Kornev Viacheslav Zhuravlev
• 来源期刊: 健康科学：英文版
• 学科: 医学
• 关键词: Molecular BIOLOGICAL methods real-time POLYMERASE CHAIN reaction RESPIRATORY tuberculosis children.
• 年，卷（期）: 2014,（5）
• 页码范围: 248-251
• 页数: 4页
• 分类号: R161

摘要

The pattern of clinical forms of respiratory tuberculosis in children shows a preponderance of intrathoracic lymph node tuberculosis （89.4%） that is characterized by a complicated process in every third child under present-day conditions. Positive result of PCR closely correlates with the severity and extent of the specific process in children. Real-time PCR （RT-PCR） was ascertain to exhibit the highest sensitivity in detecting Mycobacterium tuberculosis DNA in children with primary generalized tuberculosis （62.5%） and in those with a disseminated specific process （55.6%）, which was much higher than conventional bacteriological study of diagnostic materials. By taking into account the findings, the RT-PCR detection of M. tuberculosis was considered as a substantial criterion for evaluating the magnitude of specific changes and the degree of tuberculosis infection activity in children.