评估在里面紫外 A/ 核黄素的 vitro 抗菌剂性质调停了 Candida albicans 和镰刀霉 solani 上的 crosslinking

• 作者: Bing Sun Guo-Ying Mu Hai-Qun Yu Xiang-Chen Tao Yong Zhang Zhi-Wei Li
• 来源期刊: 国际眼科杂志：英文版
• 学科: 医学
• 关键词: antimicrobial CROSSLINKING FUNGAL RIBOFLAVIN ultraviolet A
• 年，卷（期）: 2014,（2）
• 页码范围: 205-210
• 页数: 6页
• 分类号: R772.21

摘要

AIM:To evaluate the antimicrobial properties of ultraviolet A(UVA)(365 nm)/riboflavin against Candida albicans and Fusarium solani.METHODS:Two fungus isolates were cultured in vitro and prepared with 10-fold serial PBS dilutions of cell concentration.For each dilution of fungus suspension,the concentration(colony-forming units/mL,CFU/mL)and the inactivation ratio of fungal cells were evaluated under 4 conditions:no treatment(control),UVA(365 nm)/riboflavin,riboflavin,and UVA(365 nm).RESULTS:The cell concentration decreased in UVA(365 nm)/riboflavin group for Candida albicans at each dilution and Fusarium solani at dilutions of 104,103,102CFU/mL,when compared with that in control,riboflavin,and UVA(365 nm)groups(P【0.01).No difference of cell concentration was detected amongst the culture of control,riboflavin,and UVA(365 nm)groups for the two fungus.There is a negative correlation between suspension concentration(log-transformed)and the inactivation ratio in UVA(365 nm)/riboflavin group for Candida albicans and Fusarium solani(P【0.01).CONCLUSION:According to the standard protocol of corneal collagen cross-linking,UVA(365 nm)/riboflavin combination treatment is found to moderately inactivate the viability of Candida albicans and Fusarium solani in vitro.The inactivation ratio was found to increase withthe decrease of cell concentration under UVA(365 nm)/riboflavin condition.