Generating rats with conditional alleles using CRISPR/Cas9

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2014,（1）
• 页码范围: 122-125
• 页数: 4页
• 语种: 英文
• DOI: 10.1038/cr.2013.157

摘要

Dear Editor, The rat is an important laboratory model and has many advantages over mouse models especially in toxicology and pharmacology studies.Several genome-editing technologies,such as zinc-finger nucleases (ZFNs) [1-2],transcription activator-like effector nucleases (TALENs)[3] and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated 9 (Cas9) system [4-5],have been used to produce knockout rat models by generating DNA double-strand breaks (DSBs)followed by non-homologous end joining (NHEJ)-mediated repair.However,the simple knockout strategies have limits for studying genes that are critical for embryogenesis.