Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2014,（1）
• 页码范围: 126-129
• 页数: 4页
• 语种: 英文
• DOI: 10.1038/cr.2013.156

摘要

Dear Editor, We previously developed a novel paradigm of cell activation and signaling-directed (CASD) lineage conversion for direct reprogramming of fibroblasts into cardiac,neural and endothelial precursor cells.This method is based on the transient overexpression of iPSC factors (cell activation,CA) in conjunction with lineage-specific soluble signals (signaling directed,SD).Such a strategy was used to generate human induced neural stem cells (hiNSCs) with 4 to 6 pluripotency factors,including OCT4,SOX2,KLF4 and microRNAs,from cells in human urine [1] and fibroblasts [2].Using a different strategy,SOX2 was found to induce the generation of hiNSCs,although only from fetal fibroblasts with low efficiency and by a tedious process [3].