3-54 DNA-PKcs Deficiency Inhibits Glioblastoma Cell-Derived Angiogenesis after Ionizing Radiation

• 作者: Liu Yang Liu Yuanyuan Zhang Hong Zhang Luwei
• 来源期刊: 中国科学院近代物理研究所和兰州重离子研究装置年报：英文版
• 学科: 物理学
• 关键词: DEFICIENCY INHIBITS GLIOBLASTOMA
• 年，卷（期）: 2014,（1）
• 页码范围: 148-149
• 页数: 2页
• 分类号: O4

摘要

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) plays a critical role in non-homologous end-joiningrepair of DNA double strand breaks (DSB) induced by ionizing radiation (IR) [1]. Little is known, however, regardingthe relationship between DNA-PKcs and IR induced angiogenesis; thus, in this study we aimed to further elucidatethis relationship. Our findings revealed that lack of DNA-PKcs expression or activity sensitized glioma cells toradiation due to the defective DNA DSB repairs and inhibition of phosphorylated Akt Ser473. Moreover, DNAPKcsdeficiency apparently mitigated IR-induced migration, invasion and tube formation of human microvascularendothelial cell (HMEC-1) in conditioned media derived from irradiated DNA-PKcs mutant M059J glioma cells orM059K glioma cells that have inhibited DNA-PKcs kinase activity due to the specific inhibitor NU7026 or siRNAknockdown(Fig. 1).