Complete decoding of TAL effectors for DNA recognition

• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2014,（5）
• 页码范围: 628-631
• 页数: 4页
• 语种: 中文
• DOI: 10.1038/cr.2014.19

摘要

Dear Editor, The most striking feature of a transcription activa tor-like effector (TALE) is the presence of a central DNA-binding region composed of tandem repeats of about 34 amino acids [1].Two hypervariable residues at positions 12 and 13 (repeat-variable diresidues or RVDs) in each repeat bind to DNA,and this modular DNA-binding feature of TALE repeats has inspired the development of custom-designed TALE repeats for gene editing [2,3,4,5].The nucleotide recognition preference of the commonly used RVDs has been experimentally or computationally determined [2,5].