In vivo analysis of intestinal permeability following hemorrhagic shock

• 作者: Erik B Kistler Marisol Chang Michael Richter Rafi Mazor Tom Alsaigh
• 来源期刊: 世界重症医学杂志
• 学科: 医学
• 关键词: Small BOWEL ISCHEMIA HEMORRHAGIC shock PEPTIDES MICROCIRCULATION PROTEOLYSIS
• 年，卷（期）: 2015,（4）
• 页码范围: 287-295
• 页数: 9页
• 分类号: R

摘要

AIM:To determine the time course of intestinal permeability changes to proteolytically-derived bowel peptides in experimental hemorrhagic shock.METHODS:We injected fluorescently-conjugated casein protein into the small bowel of anesthetized Wistar rats prior to induction of experimental hemorrhagic shock.These molecules,which fluoresce when proteolytically cleaved,were used as markers for the ability of proteolytically cleaved intestinal products to access the central circulation.Blood was serially sampled to quantify the relative change in concentration of proteolytically-cleaved particles in the systemic circulation.To provide spatial resolution of their location,particles in the mesenteric microvasculature were imaged using in vivo intravital fluorescent microscopy.The experiments were then repeated using an alternate measurement technique,fluorescein isothiocyanate(FITC)-labeled dextrans 20,to semi-quantitatively verify the ability of bowel-derived low-molecular weight molecules(<20 kD)to access the central circulation.RESULTS:Results demonstrate a significant increase in systemic permeability to gut-derived peptides within 20 min after induction of hemorrhage(1.11±0.19 vs 0.86±0.07,P<0.05)compared to control animals.Reperfusion resulted in a second,sustained increase in systemic permeability to gut-derived peptides in hemorrhaged animals compared to controls(1.2±0.18 vs 0.97±0.1,P<0.05).Intravital microscopy of the mesentery also showed marked accumulation of fluorescent particles in the microcirculation of hemorrhaged animals compared to controls.These results were replicated using FITC dextrans 20[10.85±6.52 vs 3.38±1.11 fluorescent intensity units(×105,P<0.05,hemorrhagic shock vs controls)],confirming that small bowel ischemia in response to experimental hemorrhagic shock results in marked and early increases in gut membrane permeability.CONCLUSION:Increased small bowel permeability in hemorrhagic shock may allow for systemic absorption of otherwise retained proteolytically-generated peptides,with